At this point you can either leave the CPU and memory on the old board or remove them outright, but the AGP, PCI, and other peripheral cards should be removed at once. Place all components in or on the anti-static bag for safety and store them well out of harm's way. Then locate the motherboard's mounting screws and remove them as well, being careful to place them safely in a container afterward. Do one last check to ensure that no cables are attached to the board, since missing even one may result in ripped cables or bent jumpers while removing the motherboard.

Then remove the motherboard itself, keeping in mind that the board will need to slide forwards a bit to disengage the ATX backplate connectors. The motherboard should come off smoothly, and if it needs to be forced, you either missed a mounting screw or the ATX connectors are still inserted in the case backplate. After it is removed, place the motherboard back into its original box, or some other packaging of your choice. It helps cut down on confusion to distance the old motherboard, and its documentation and drivers, from the work area before physically installing the new motherboard.